Centrobin on Brain tissue?

10 November 2016 1,966 1 View

I am looking for an antibody of pax6 and Tbr2 that are not made in rabbit?

I would like to make a double staining Pax6/Tbr2, but the antibodies for pax6 and Tbr2 that we have in the lab are both made in rabbit. Before I was using a mouse anti Pax6 but the manufacturer...

02 March 2015 8,739 4 View

I am looking for some manufacturers which can produce specific antibodies. Can someone help?

I wood like to study the NHEJ mechanism and especially the molecular mechanism involving the gene Cernunnos. Cernunnos is also named NHEJ1 or XLF in human. I have already bought an antibody...

02 March 2015 6,101 6 View

Do you have experience with the p21 promoter from a mouse for GFP construct ?

I would like to generate a GFP vector under the control of p21 promoter from mice. Does anyone know whether such a vector exists? Does anyone know whether someone has already tried to get the p21...

06 July 2014 6,043 4 View

Immunofluorescent labeling

I am looking for various antibodies in order to label cortical slices of mouse embryos. Those antibodies are anti-Gsh2 (or gsx2), anti-Emx1, anti-EGFR, anti-Egr1, anti-TGFalpha.

08 September 2013 5,584 2 View

Anti-p21 antibody to label cortical neural stem cells?

I would like to label p21 positive cells in irradiated mouse embryonic cortex. I am currently using an antibody from Cell signaling that is directly coupled with the Alexa Fluor 488 (Ref 5487)....

07 August 2013 6,927 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View