I would like to generate a GFP vector under the control of p21 promoter from mice. Does anyone know whether such a vector exists? Does anyone know whether someone has already tried to get the p21 promoter from mouse genome?
There is one paper that indicates they used such a construct, but I don't see precise details in the methods:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0007469
There are some commercial offerings like this one:
http://www.genecopoeia.com/product/search/detail.php?prt=22&cid=&key=MPRM13359
Some papers like this one describe p21 promoter driven luciferase constructs:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019278/
The mouse p21 promoter is a highly characterised element due to its involvement in p53 functionality so there will be plenty of vectors containing a variety of p21 promoter sequences. If you are struggling to find a GFP/ EGFP reporter specifically, through a search on pubmed, you can look for a p21-Luciferase vector, and request this vector from the lab, and remove the p21 promoter from this and replace into a GFP vector yourself.
Thanks for your answers.
However these promoters are all from human p21 et not from mouse.
Thanks
I presume you are still looking for this, so this will help.
p21 was called WAF1 when it was first cloned, which may be why you struggle to find this construct. It was first identified by Wafik El-Deiry when he was in Bert Vogelstein's lab. This is the paper that described it. They cloned the promoter into a luciferase vector to look at it's activation by p53. Plasmid construction is in the paper and Addgene sell the plasmid.
http://www.cell.com/cell/pdf/0092-8674(93)90500-P.pdf
https://www.addgene.org/16451/
However, the names I gave have continued to work in this field so searching them on pubmed may show that they later cloned this promoter into a GFP vector, or you could try emailing them to ask. Although, bear in mind that busy professors are more likely to help if you can identify a specific vector that they have published and ask for it by name since they are only obliged to supply plasmids they have published.
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