Centrobin on Brain tissue?

10 November 2016 1,966 1 View

I am looking for an antibody of pax6 and Tbr2 that are not made in rabbit?

I would like to make a double staining Pax6/Tbr2, but the antibodies for pax6 and Tbr2 that we have in the lab are both made in rabbit. Before I was using a mouse anti Pax6 but the manufacturer...

02 March 2015 8,739 4 View

I am looking for some manufacturers which can produce specific antibodies. Can someone help?

I wood like to study the NHEJ mechanism and especially the molecular mechanism involving the gene Cernunnos. Cernunnos is also named NHEJ1 or XLF in human. I have already bought an antibody...

02 March 2015 6,101 6 View

Do you have experience with the p21 promoter from a mouse for GFP construct ?

I would like to generate a GFP vector under the control of p21 promoter from mice. Does anyone know whether such a vector exists? Does anyone know whether someone has already tried to get the p21...

06 July 2014 6,043 4 View

Can you suggest some good molecular markers for cortical layers in postnatal and adult mice?

I am looking for antibodies against RORbeta, CALB1 and PCP4. I would like to know if someone has ever used any of these antibodies? Where did you buy the antibodies? How do they work on mouse brain?

05 June 2014 943 0 View

Anti-p21 antibody to label cortical neural stem cells?

I would like to label p21 positive cells in irradiated mouse embryonic cortex. I am currently using an antibody from Cell signaling that is directly coupled with the Alexa Fluor 488 (Ref 5487)....

07 August 2013 6,927 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View