Immunofluorescent labeling

More Amandine Bery's questions See All

Centrobin on Brain tissue?

I am looking for someone who has already used an anti-centrobin antibody to label centrioles on brain tissues?

10 November 2016 2,013 1 View

I am looking for some manufacturers which can produce specific antibodies. Can someone help?

I wood like to study the NHEJ mechanism and especially the molecular mechanism involving the gene Cernunnos. Cernunnos is also named NHEJ1 or XLF in human. I have already bought an antibody...

02 March 2015 6,139 6 View

I am looking for an antibody of pax6 and Tbr2 that are not made in rabbit?

I would like to make a double staining Pax6/Tbr2, but the antibodies for pax6 and Tbr2 that we have in the lab are both made in rabbit. Before I was using a mouse anti Pax6 but the manufacturer...

02 March 2015 8,780 4 View

Do you have experience with the p21 promoter from a mouse for GFP construct ?

I would like to generate a GFP vector under the control of p21 promoter from mice. Does anyone know whether such a vector exists? Does anyone know whether someone has already tried to get the p21...

06 July 2014 6,083 4 View

Can you suggest some good molecular markers for cortical layers in postnatal and adult mice?

I am looking for antibodies against RORbeta, CALB1 and PCP4. I would like to know if someone has ever used any of these antibodies? Where did you buy the antibodies? How do they work on mouse brain?

05 June 2014 980 0 View

Anti-p21 antibody to label cortical neural stem cells?

I would like to label p21 positive cells in irradiated mouse embryonic cortex. I am currently using an antibody from Cell signaling that is directly coupled with the Alexa Fluor 488 (Ref 5487)....

07 August 2013 6,971 6 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View