Centrobin on Brain tissue?

Amandine Bery @Amandine_Bery

11 November 2016 1 2K Report

I am looking for someone who has already used an anti-centrobin antibody to label centrioles on brain tissues?

Vladimir A. Kulchitsky

I can only help two scientific articles on the subject (in the annex to the letter). All the best.

Badges
Science topic

More Amandine Bery's questions See All

I am looking for some manufacturers which can produce specific antibodies. Can someone help?

I wood like to study the NHEJ mechanism and especially the molecular mechanism involving the gene Cernunnos. Cernunnos is also named NHEJ1 or XLF in human. I have already bought an antibody...

02 March 2015 6,139 6 View

I am looking for an antibody of pax6 and Tbr2 that are not made in rabbit?

I would like to make a double staining Pax6/Tbr2, but the antibodies for pax6 and Tbr2 that we have in the lab are both made in rabbit. Before I was using a mouse anti Pax6 but the manufacturer...

02 March 2015 8,780 4 View

Do you have experience with the p21 promoter from a mouse for GFP construct ?

I would like to generate a GFP vector under the control of p21 promoter from mice. Does anyone know whether such a vector exists? Does anyone know whether someone has already tried to get the p21...

06 July 2014 6,083 4 View

Can you suggest some good molecular markers for cortical layers in postnatal and adult mice?

I am looking for antibodies against RORbeta, CALB1 and PCP4. I would like to know if someone has ever used any of these antibodies? Where did you buy the antibodies? How do they work on mouse brain?

05 June 2014 980 0 View

Immunofluorescent labeling

I am looking for various antibodies in order to label cortical slices of mouse embryos. Those antibodies are anti-Gsh2 (or gsx2), anti-Emx1, anti-EGFR, anti-Egr1, anti-TGFalpha.

08 September 2013 5,616 2 View

Anti-p21 antibody to label cortical neural stem cells?

I would like to label p21 positive cells in irradiated mouse embryonic cortex. I am currently using an antibody from Cell signaling that is directly coupled with the Alexa Fluor 488 (Ref 5487)....

07 August 2013 6,971 6 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can telekinesis, telepathy, and prediction be achieved through science?

Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...

28 July 2024 7,522 0 View