Did anyone have any troubles while performing acetic acid induced writhing test in mice?

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Tejas Ganatra

Two main difficulties:

1. Mortality rate

2. Perfect recognization of Writh (False positive)

To overcome these, I prefer to use Eddy's hot plate or Tail flick apparatus to check analgesic acitivity!!!

Nikola M Stojanovic

Thank you for your answer but still i have difficulties:

1. What do you mean under morality rate?

2. We recognize a writh as specifically described contortion of body (in each reference using this assay is stated)

As for HP and TF test those are used for detecting the analgesic activity of compound in CNS not in PNS. Thus the aceti acid induced writhing is needed!