Can you suggest me fluorimetric method for saliva cortisol determination?

I have trouble finding adequate method for fluorimetirc determination of saliva cortisol. I don't want to use ELISA nor RIA. Can you help me? Also, do I have to extract cortisol from saliva? How...

05 June 2016 8,756 3 View

Can you suggest me the protocol for XTT assay?

31 December 2014 4,813 5 View

Can anyone help me with cell isolation and culturing?

I have some experience in cell isolation and culturing them. However those are mostly short assays (1-2h) and no real stuff. I want to know what culture medium is best for macrophages,...

08 September 2014 6,431 20 View

Behavior asymmetry coefficient?

07 August 2014 2,682 10 View

Acetic acid induced writhings in mice?

I asked similar question some time ago and i want to try to get the help once more! During experiments with abdominal writhing induced with acetic acid very different results are obtained! In the...

07 August 2014 3,755 5 View

Does anyone have mouse brain atlas by Paxinos and Franklin (2001)?

06 July 2014 8,557 55 View

Can someone suggest method for determination of oxidised glutathione ?

But if it is possible spectrophotometric (not fluorescence)

04 May 2014 7,367 2 View

How to prepare a solution of silymarin suitable for i.p. application to rats?

What solvent, solution? What dose?

02 March 2014 2,410 4 View

Picrotoxin convulsions?

Could you suggest doses for induction of convulsions with picrotoxin?

11 December 2013 6,284 4 View

Did anyone have any troubles while performing acetic acid induced writhing test in mice?

Very different number of writhings among animals in group

11 December 2013 9,017 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View