Can someone suggest method for determination of oxidised glutathione ?

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Adam B Shapiro

Is there also reduced glutathione present? If not, try reducing the oxidized glutathione with TCEP, then measuring the free thiol concentration with Ellman's reagent by 412 nm absorbance. I haven't tried this, so I don't know whether the TCEP will interfere with Ellman's reagent. If you try it, let me know what happens.

Nikola M Stojanovic

I have already measured reduced glutathione now i need oxidised form. i haven't done the method that you suggest so far and I doubt we have TCEP and its not easy to obtain chemicals in Serbia....