In relation to your experience what is the best Ovarian Cell Line to work with?
Thank you!
Dear All, here is my question: if I have a 15-scans cyclic voltammetry performed with PalmSens, how can I obtain the charge passed during the process? Hopefully avoiding integrations,...
29 July 2024 3,733 5 View
Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...
18 July 2024 382 5 View
Dear all, I'm currently working with CHI potentiostat and performing Impedance. However, for the circuit fitting the instrument must be connected to the computer. Since I prefer to analyse the...
10 July 2024 8,880 4 View
Hello to everyone, I am trying to launch a BLYP optimization with ORCA starting from an already converged calculation at BP level ,exploiting its orbitals and geometry as a guess. I tried...
24 June 2024 3,768 2 View
A binary classifier based on multivariate Gaussian models, which estimates the mean vector and the variance-covariance matrix during the training phase and returns the class with the highest...
23 June 2024 10,114 1 View
Dear all, I found in cell cultures some structures that I cannot identify I will really appreciate is someone would give me some suggestions Many thanks Francesco
09 May 2024 2,070 11 View
Hello everybody. For my project i need to identify the APOe isoforms present on my patient derived lines. My idea was to extract DNA, amplify with PCR and send it for Sanger sequencing -...
24 April 2024 6,359 4 View
What should we do with high-correlation data? Did we remove them, or do they not matter? WHY?
12 April 2024 9,429 3 View
Greetings. In the context of the new ISO 14068 on Carbon Neutrality, how is double-counting of emissions between two subjects been dealt with? Suppose Company B is assessing the carbon neutrality...
03 April 2024 3,596 1 View
Is there any standardised index to estimate, more or less precisely, muscle mass from body weight (e.g., Bodyweight * index)? Any reading suggestions are much appreciated.
27 February 2024 8,285 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View