Im currently working on a project to quantify Il-1beta increases following a bout of eccentric downhill running. My adviser recommended finding a kit used in the literature that allows for practice. Im having a difficult time finding specific information on the kits used in the papers Ive been reading. Would appreciate some suggestions from people that have measured Il-1beta.
Hi Kyle,
In my opinion, to quantify the levels of L-1 beta in urine, ELISA is the best method. Many companies provide ELISA kits, but you have to check whether the selected kit quantify the cytokine level in your sample (urine).
Regards
Trace proteins in body liquids with low protein concentrations should be stabilized pre-analytically.
Add 1 part of your sampled urine to 1 part of 5 % human albumin of drug quality. You might then add 1 part of this mixture to 1 part of 2.5 M arginine, pH 8.6. These aliquots are stable against artefactual changes such as induced by freezing/thawing.
Be sure that your EIA-standards are treated similarly.
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