Can anybody provide me sequences of SPF10 primers?

03 February 2021 1,053 2 View

How to input an exogenous variable in a GARCH model?

Hello, I have a question regarding financial time series. I am studying the effects of Quantitative Easing on the US stock market. I have tried to fit an ARIMA model to my S&P500 returns with...

01 February 2021 8,325 3 View

Kruskal-Wallis Test does not offer results in Model Viewer (SPSS 26.0)?

One of my students faces this problem in SPSS 26.0: When he runs Kruskal-Wallis test, the Output does not offer him access to the Model Viewer (where the pairwise comparison etc. would be...

11 November 2020 6,345 8 View

How to model utility grid in solar grid tied system in Simulink?

I am modelling grid tied solar PV with backup battery and I inserted load between solar system and grid. According to the graph of rms currents: Igrid = Ipv + Iload, which doesnt make any sense to...

10 May 2018 4,694 2 View

SPSS error "subscript is out of range", what to do?

21 January 2018 9,637 4 View

How to explain different patterns resulting after acridine orange assay for assessment of sperm DNA integrity?

We have performed AO assay on human ejaculated sperm. Everything seems, that works well, however we observed und microscope every cells stainned green and moreover there are two distinct...

02 October 2017 3,273 3 View

Method to make thin layer on glass substrate from nanopowder scintillator

Hi, does anyone have experience with making a thin film from scintillator nanopowder or nanopowder in general (GGAG:Ce, LuAG:Ce ... 10 - 30 nm particle size) on glass substrate. I writing master's...

14 August 2017 1,964 1 View

Does anyone experience with sonication of HCT116 on Bioruptor Pico?

17 July 2017 9,180 1 View

Is it the same RNase inhibitor for blood plasma as for RNA isolation?

02 September 2015 3,872 1 View

How do I decrease protein expression from pETDuet vector?

08 February 2015 7,968 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View