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Questions related from N.Subash Chandra Bose Raju
I wish to know about the reason behind getting VFA values in negative. Initially for few days, I got positive values (91mg/L to 13.61 mg/L) and for the past two days my values went to -20.09 and...
10 October 2018 5,846 2 View
I have carried out scanning Electron Microscope studies Thrips ( Name cannot be revealed at this moment). The characters are well studied in few reports but SEM studies not so far.Along with SEM...
05 May 2015 8,309 2 View
I have found out following strains from insect gut flora Devosia chinhatensis, Pseudomonas stutzeri, Serratia nematodiphila through 16sRNA analysis. There are many reports about these strains at...
03 March 2015 5,829 4 View
We have carried out gutflora analysis of H.armigera and found with many isolates including Bacillus pumilus ,B.safensis, D,chinhatensis ,P.stutzeri, S.marscens, S.nemadophilia, S.maltophila. I am...
02 February 2015 5,782 3 View
I found the genus Fulmekiola (Thrips) a few days ago and am unable resolve it at the species level. Although a few papers are available, I am unable to find dichotomous keys for this genus. I...
12 December 2014 9,889 3 View
I have analyzed gut flora of Helicoverpa and found B. pumilius, with some strain identification by 16S rDNA analysis. I want to check these strains via nucleotide diversity by DNA sp software. I...
12 December 2014 9,790 8 View
I am looking for a good vector for the cloning of gut-micflora metagenome. The source of metagenome is insect gut. I isolated a number of cultures and did 16sRna sequencing. On the other side we...
01 January 2014 5,714 6 View
Can someone assist me regarding the analysis of 16sRNA sequences. After my PCR, I sent my number of samples for sequencing. I got report from them as Forward Trimmed sequence and Reverse trimmed...
12 December 2013 9,174 4 View
I digested pcr products by MboI enzyme, expected 2 bands one at 296 and another at 72bp. I got respectively in few samples which means they are resistant, the wild type will not show any cut...
11 November 2013 1,072 6 View