Which antibody do you recommend to assess Fyn kinase phosphorylation and activation?
I have seen several publications using this one Phospho-Src Family (Tyr416) Antibody #2101 from Cell Signaling but this also recognizes all other members of the Src family.
Attaching a paper who have used pFYN from Santa Cruz, for microscopy.
Hope this will work.
Andreia, there are 2 "problems"
1) the phosphorylation occurs at a motif that is recognized by a kinase that can phosphorylate several proteins
2) phosphotyrosine itself is a pretty prominent antigen (see e.g. the famous PY20 antibody), so generating antibodies against phosphotyrosine motifs is quite difficult.
Thus it's very unlikely to find an antibody that will specifically recognize pFyn at this site. If you're doing Westerns, you may either probe for Fyn, too, to identify the corresponding protein or enrich it from a sample by immunoprecipitation. In IHC, co-staining for Fyn with a different colour should do it.
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