What is the best method for quantifying lipid accumulation in C. elegans images using ImageJ?

16 April 2024 1 2K Report

We have a demarcation and analysis protocol in ImageJ for images of C. elegans stained with Oil Red O. However, it does not seem to be the best way and we were unable to find an easy-to-execute protocol.

A.B Bayazid

Dear Ms Isamara Carvalho

How about after

Oil red O of and DW washing, Add 100% isopropanol and incubate 10 min for dissolve Oil Red O.

Collect the isopropanol in e-tube

Measure OD at 500nm.

Kind regards

AB Bayazid

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