Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Does anyone know one antibody that works against Mycobacterium spp?

Anti-Mycobacterium antibody: does anyone know one antibody that works against Mycobacterium spp. (mainly for immunofluorescence)? Many thanks in advance! Cristiane

04 January 2021 9,724 3 View

Can likert-type statements formulation derive solely from literature review?

28 October 2020 9,951 3 View

How to report analytical uncertainty calculations in a research paper about environmental contamination?

We submitted a paper investigating trace elements levels in multiple abiotic and biotic matrices using analytical protocols already established. One of the reviewers required us to report...

27 October 2020 8,453 4 View

Does 2-methoxyethanol reacts with air?

Hello, I'm trying to use 2-methoxyethanol/Cellosolve to dilute methylumbelliferyl phosphate for phosphatase activity analysis. However, we never used this solvent before. It came with a sure/safe...

27 October 2020 7,302 1 View

Can we predict differential expression genes (MicroRNAs) by analyzing a DNA Methylation dataset?

29 July 2020 7,333 2 View

What is the best protocol for evaluate TMR-peptide?

I apologize if my question seems to naive, but I would like to do a protocol to determine bio-distribution of a peptide coupled to TMR in an animal model however, I am having some difficulties in...

07 July 2019 5,664 2 View

Anyone could give some tips for fish brain microdisection?

I want to isolate fish brain specific zones like telencephalon, cerebelum, opticum tectum. I am encoutering with the problem of hardness of tissue because in the moment we try to take fish brain...

30 April 2019 8,841 1 View

In which countries of the world have digital textbooks been used as a substitute for printed models?

I think the digital textbook is an important additional resource to the printed book for enabling portability and choices for the student.

21 March 2019 9,022 6 View

How can you read cel files using the R program?

27 February 2019 8,863 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View