I have already carried out the lipid staining test in C. elegans with oil red, however, in the last tests I am unable to stain all the animals efficiently, most of them do not stain or only have part of the lipid droplets stained.
I don't know where I'm going wrong in the protocol, maybe when preparing the 0.3% oil red solution in 60% isopropanol.
Hi Isamara, in our lab we follow the protocol for single samples as described in this paper "Wählby C, Conery AL, Bray MA, Kamentsky L, Larkins-Ford J, Sokolnicki KL, Veneskey M, Michaels K, Carpenter AE, O'Rourke EJ. High- and low-throughput scoring of fat mass and body fat distribution in C. elegans. Methods. 2014 Aug 1;68(3):492-9. doi: 10.1016/j.ymeth.2014.04.017. Epub 2014 Apr 28. PMID: 24784529; PMCID: PMC4112171." They also use isopropanol 60% to fix the worms but the ORO is 0,5% prepared in isopropanol 100%. We did this way a few times and it worked very well.
- Badges
- Science topic
- Similar topics
- Filing