Patrick Connolly

5 Questions 3 Answers 0 Followers

Questions related from Patrick Connolly

Designing a pharmacophore model of a protein-protein interaction hotspot with known essential residues?

Hello structural biologists! I am interested in developing an inhibitor to target the assembly of a specific heteromultimeric complex. The complex exists in the cytoplasm of most eukaryotic cells....

03 May 2018 7,011 5 View

Can you immortalize murine cells with human TERT?

I am looking to immortalize skeletal and cardiac myoblasts without messing with p53. Skeletal myoblasts can be readily transformed with a combination of mTERT and CDK4 (I suspect that this will...

17 July 2017 8,296 3 View

What is the 'gold standard' method to estimate the number of DNA strand breaks in a nucleus (or the average number per nucleus in a population)?

What is the 'gold standard' method to estimate the number of DNA strand breaks in a nucleus? (or the average number per nucleus in a population)

25 September 2016 5,836 1 View

Can H2AX phosphorylation be used as a marker for strand breaks with overhangs, or does it only respond to blunt strand breaks?

I am attempting to analyze nuclear DNA damage induced by an ectopically-expressed endonuclease. This nuclease recognizes an eight-base sequence, and forms a double-stranded break with a two-base...

26 August 2016 992 2 View

How do I calculate the W/cm^2 received by the surface of a tissue culture hood when irradiated by a 30W UV lamp at 90cm distance?

The lamp in question, in case more information is...

01 July 2016 5,293 6 View