Hi Azmir

first, I am not expirienced with plasma cell miRNA preparation.

It seems you have a relatively low yield of RNA (which might be normal for plasma). add the limits of the nanodrop, and you come to the conclusion that it is hard to judge too much from these pictures.

Therefore, minor residues of proteins or salt can lead to the low 260/280 and 260/230 values.

Your high 230 values indicate resiudes of ethanol or chaotropic salts from the extraction kit, which regularily occur with these kits.

I would not be too preoccupied about the values and try to continue with your RNA... maybe you could try to improve your concentration of RNA (more strating material or less elution volume).

Best

Christian

Thank you Christian for your helpful answers. Do you mean that I should try to continue with qPCR? For your information, these extract will be used in qPCR to observe the plasma miRNA expression.

This is my PhD project. If in viva session later, if the panel ask me about the quality of my extract, how should I answer it? Do you have any alternative quality checking test to do for such extracts so that I can defend during my viva later?