Camilla Romagnoli

3 Questions 2 Answers 0 Followers

Questions related from Camilla Romagnoli

P21 molecular weight?

I'm working on senescence, and I'm trying to observe senescence markers. I have performed western blots for p21 but it seems that the bands are at a much higher molecular weight than expected. The...

23 March 2024 9,238 2 View

Can you culture HUVECs with DMEM ?

Hi, I am culturing HUVECs using the Endothelial Growth Media from PromoCell, however is pretty expensive. Does anyone know any alternative? is it possible to use DMEM for culturing HUVECs?

13 September 2023 9,465 1 View

Which are the best cytokines to use as markers for the senescence associated secretome phenotype (SASP)?

Hello, I'm working on senescence and I'm inducing it using TNF alpha. I would like to assess the induction of the phenotype looking for the cytokines typical of the secretome. Which cytokines do...

20 September 2022 2,217 3 View