I am trying to work out the growth rate of yeast cells based on OD measurements. I understand the formula for specific growth rate is U= (ln (x/xo))/t but can you apply this formula for measurements based on OD?
Hi there,
Definitely yes if your OD measurements are strictly proportional to the density/number of cells present in the sample. To get sure of that OD value has to be within the linearity domain of the spec you use (usually OD has to be under 0.5) and cell sample has to be homogenous when measuring OD. In the formula you replace x and x0 by OD at t and OD at t0 respectively.
Hi there,
actually, you can easily work out the OD range where your photometer produces reliable readings. Just take a well grown yeast culture and make 2-fold serial dilutions of it.
First measure the most dilute suspension and note the OD. Continue with the more concentrated suspensions. In this approach, every OD reading should be twice the value of the previous reading. The point where this is no longer true is the upper limit of the range your photometer can be used.
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