In what deeper sense can you speak about the quasiclassicality of the DNA macromolecule?

You can see under an ordinary light microscope that a DNA polymer is nearly a macroscopic object. But in a strict quantal sense can we expect quasiclassical behaviour, i.e., describability by a...

03 February 2017 7,734 2 View

Which value of RNA Integrity Number (RIN) is considered suitable/sufficient for qPCR?

Which value of RNA Integrity Number (RIN) is considered suitable/sufficient for qPCR? Thank you

21 April 2016 2,021 3 View

Any advice in choosing MoBio PowerSoil vs. PowerMax Soil DNA Isolation Kit for fungal pyrosequencing?

We have sandy soil samples with quite patchy fungal communities. At least I got a bit different ITS T-RFLP profile for three parallel PowerSoil DNA samples. And when I made a fourth isolation with...

12 December 2015 3,767 6 View

Can somebody suggest the liquid mono Isopropylamine heat extension factor?

Can you tell me somebody the liquid mono Isopropylamine cubical expansion coefficient?

06 October 2015 856 1 View

Single Primer PCR

Hello everyone, Has anyone successfully performed single strand PCR for sequencing? In essence, I have extracted a genome from a stable transfectant I created. But I'm not sure where the gene...

03 May 2011 4,078 1 View

Best Techniques for detachment, collection, and replating cells

Hi, I have a 3T3 cell line that I am trying to make into a permanent transfectant. The first part of the protocol involved a harsh antibiotic screen that left only a few cells in the dish to...

30 November 2009 7,183 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View