Which value of RNA Integrity Number (RIN) is considered suitable/sufficient for qPCR?

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Can you adjust MiniAmp Thermal cycler cover gradient?

I was wondering if anyone had issues with their MiniAmp. Overall, I'm quite happy with the instrument but I find the lid heats/cools extremely slowly (0.3 degrees/sec) and I cannot find any way to...

07 July 2022 7,476 0 View

RT-PCR systems for SARS-CoV-2 identification: SLAN 48p vs. AGS 4800?

Hi, Everybody, I'm looking for someone with expertise with Zeesan's Lab-Aid 824s Extraction System and/or SLAN®- 48P RtPCR (especially with the SARS-CoV-2 kit). What are your in-practice...

08 May 2020 8,246 0 View

How to obtain similar specific growth rate value of linearisation with modified Gompertz model fitting?

I am not satisfied with the method of linearisation of microbial growth curve to calculate the maximum specific growth rate (ln(x1-x0)/(t1-t0), because to find the linear part of the ln curve is...

06 December 2019 6,149 4 View

What is the ratio of 3D/3+2D/5D milling technology?

I am looking for a survey or report about the application ratio of 3D/3+2D/5D milling technology in the industry.

18 December 2018 7,021 2 View

Modelling predator-prey interactions

We intend to explore predation rates on snakes by using clay models but "ordinary" plasticine melts when exposed to heat. Can anyone recommend a suitable brand of modelling clay that does not lose...

14 August 2017 1,878 4 View

Would you have any idea how to transform the wavefunction as such into an informational onservable in quantum algebraic sense?

I am contemplating about such a formalism as regards to introduce the wave side of the dualism in a much more explicite way than is done in orthodox QM, in which the "informationally"...

03 February 2017 2,280 22 View

In what deeper sense can you speak about the quasiclassicality of the DNA macromolecule?

You can see under an ordinary light microscope that a DNA polymer is nearly a macroscopic object. But in a strict quantal sense can we expect quasiclassical behaviour, i.e., describability by a...

03 February 2017 7,868 2 View

Which types of natural clays and zeolites are the most effective adsorbents for phenol and PAH?

Many researchers agree that chemically modified clays and synthetic zeolites can be useful treatment for wastewaters polluted by phenol and/or PAH compounds. But which are the best tested natural...

22 May 2016 9,098 1 View

Any advice in choosing MoBio PowerSoil vs. PowerMax Soil DNA Isolation Kit for fungal pyrosequencing?

We have sandy soil samples with quite patchy fungal communities. At least I got a bit different ITS T-RFLP profile for three parallel PowerSoil DNA samples. And when I made a fourth isolation with...

12 December 2015 3,854 6 View

Can somebody suggest the liquid mono Isopropylamine heat extension factor?

Can you tell me somebody the liquid mono Isopropylamine cubical expansion coefficient?

06 October 2015 977 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Need help with my research project on open source SIEM and machine learning?

Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...

04 August 2024 3,196 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View