Can we use HPLC for the detection of specific enzymes?

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How to design guideRNAs that should target all the alleles of a gene in monocot?

In banana, we are having multiple alleles. For distrusting the function of a gene through CRISPR/Cas9 it is important to mutate all the alleles through designing specific guideRNA repeats. How to...

15 August 2022 5,313 1 View

How to do Fluid-structure Interaction modelling of patient specific 3D arterial blood flow?

I am getting convergence issues in the first coupling iteration itself and the solver stops. I tried refining the mesh and also tried reducing the time step size. It is still not working. If...

06 July 2020 6,617 7 View

Influence of COVID on the mental health - Survey?

Dear Pls, follow the link provided below to register to invaluable experiences in the face of COVID-19. https://swinuw.au1.qualtrics.com/jfe/form/SV_88qs1TRRQAv8Q9T

14 April 2020 384 3 View

Can anyone say about to make a perfect way to make GO bases Nanocomposite?

I am working in ternary composites. Is there anyone working in graphene and its derivatives. I want to make a composite comprised of GO with some inorganic material.

05 May 2019 6,977 3 View

How to model the leakage of water from a closed container when the valve is opened?

I patched the fluid domain with 1400 gauge pressure and set up the boundary condition at the outlet to be 0 gauge pressure. But I am getting floating point exception. What am i missing?

04 April 2019 8,579 4 View

Can anyone say clearly about the calculation for doping metal oxides using some different mol ratios?

I need to dope metal to my metal oxide matrix, Can anyone clearly explain me about the calculations that i need to workout for mol %.

26 March 2019 1,470 4 View

Is there any medium to support the growth of Streptomycetes only?

Does the growth of microbes on ISP4 medium confirm the organisms to be a streptomycete? Kindly suggest some selection medium for Streptomycetes

17 February 2019 7,336 3 View

Where can can I buy Ceramic Matrix Composites (CMC) SiC-C or SiC-SiC?

Hi, Does any company in Europe sell Ceramic Matrix Composites (CMC) (SiC-C or SiC-SiC) components or flat plates. Thanks Sundar

04 February 2019 4,467 3 View

Reference required - reg?

Dear Friends, In Biodiversity Heritage Library, I couldn't download the volumes from 8 onwards. If anybody is having the following reference, kindly support me to share it. "Clark AM 1967. Notes...

02 January 2019 6,975 6 View

Air pollutant accumulated Filter paper preservation?

Usually the filter papers will be desiccated for 24 hr before and after ambient air pollutant sampling. As we encounter some error in weighing balance, we are unable to weigh the exposed air...

22 October 2018 1,925 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View