Why Marine agar turnig blue?

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Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

GDP-Bodipy assay for GTPases RhoA and Rac1, help?

I am been working for a couple of months without success on setting up an assay based on GTPases loading with bodipy gdp and then measure the exchange to GTP in presence of various GEFs. Reading...

16 March 2024 1,600 3 View

Should we teach grammar?

explicit or implicit grammar

15 February 2024 9,660 5 View

How to measure Redox Reaction with SnF2?

I have two slurries of SnF2 with / without potential stabilizer and I want to measure if the SnF2 is actually stabilized or not. I was advised to check the Redox Potential to verify the...

17 August 2023 7,715 0 View

I am conducting a systematic review on teacher resilience. Can I search articles from here but about the search string how do I actually do it?

I am conducting a systematic review on teacher resilience. Can I search articles from here but about the search string how do I actually do it and find the exact hit (a number of searches) and...

04 December 2022 2,130 5 View

Using Nanopore, how many long reads per barcode and with what determined N50 are necessary for the assembly of a microorganism?

In sequencing using nanopores by MinIon, how many long reads per barcode and with what determined N50 are necessary for the assembly of a microorganism?"

06 November 2022 8,991 0 View

How to calculate qpcr copy number per gram of soil?

Hi, I am working with qPCR data, where I independently used a standard curve to calculate the gene copy number in my soil samples as a proxy for bacteria and fungal biomass. However, I currently...

09 August 2022 5,787 0 View

What is the best software for systematic literature reviews?

Performing the research search process in a specific area is very useful to systematize scientific productivity. However, performing a systematic review without software can be a slow and...

26 May 2022 3,079 12 View

Which parameters should be evaluated to perform the Partial Validation of a chromatographic method already validated?

I have fully validated an analytical method in HPLC and now following the purchase of a new instrument (same brand new model) I will have to do the partial validation. Unfortunately, EMA does not...

07 September 2021 1,706 3 View

CellMask membrane staining for cells aspecific?

Hello, I am using thp-1 cells on a cyclic olefin support for confocal microscopy. After. 2% formaldehyde staining , three washings,1% bsa and I stain them with Cell membrane staining CellMask Deep...

14 July 2021 1,367 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,564 6 View

Which is the best media for revival of Pseudomonas aeruginosa?

Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...

09 June 2024 6,651 9 View