Hi guys! I want to extract RNA and use it for q_PCR. As it is convenient to collect ear tissue or blood samples from animal so which method will be more efficient to collect RNA from these tissues.
Thanking you in advance.
For animal blood samples, you can isolate the PBMC using centrifugation method with the aid of ficoll and then use trizol to obtain the RNA.
Thanks Zx Chong
Dr Muhammad Ameen yes you can
Yes Sir, you can.
Yes.
See here.
http://compbio.dfci.harvard.edu/compbio/research/labprotocols/contentBlocks/0/contentBlock_files/file18/Total_RNA_Extraction_from_Human_Tissue_Samples_using_TRIzol.pdf
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