Muhammad Ameen Jamal

6 Questions 7 Answers 0 Followers

Questions related from Muhammad Ameen Jamal

ELISA or RIA?? which one is more convenient and accurate for measuring serum hormonal profile in pigs??

Need recommendations please.

06 June 2019 3,209 6 View

Can I extract RNA from ear tissue or blood samples using TRIZOL or KIT?

Hi guys! I want to extract RNA and use it for q_PCR. As it is convenient to collect ear tissue or blood samples from animal so which method will be more efficient to collect RNA from these...

05 May 2019 8,034 5 View

Which method ELISA or RIA is more suitable for Hormonal analysis?

Hello All. I have to measure levels of various reprroductive hormones in serum of sow. I want to know which method is more suitable for hormonal analysis as I reviewed many articles and few used...

10 October 2018 576 0 View

How to separate Hypothalmus and lobes of pituitary ( anterior and posterior) from the brain of pig and how much sample is required for checking expr?

Hello, I was interested to work on hypothalamus and pituitary. So, I want to know how to separate hypothalamus and both lobes of pituitary from brain of piglet. Further, how much amount of sample...

06 June 2018 9,721 1 View

Need help! Why no band appear at gel?

Hi. Everyone. I have extracted DNA from hair follicles through Brief extraction buffer and measured conc. by nanodrop (100 ng/ul) but when I used this sample for PCR, no amplification observed....

03 March 2018 5,911 6 View

Can we prepare pseudopregnant mouse without breeding with male?

Dear colleagues! is it necessary to breed the female with vasectomised male for pesudopregnancy. As breeding objective is ovulation induction and preparation of uterus for carrying pregnancy. But...

02 February 2017 5,103 3 View