Can we measure pH of a solution having beta mercaptoethanol or SDS in it with help of pH meter or we should use pH strip for that?

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Do all the mRNAs of pox virus are polyadenylated at 3' end?

Hello everyone, In eukaryotic cells, all the mRNAS are polyadenylated at 3' end. I want to ask whether this is the same case for pox viruses also. Do all their mRNAs are polyadenylated at 3'end...

04 May 2019 3,309 2 View

Can we use freezed nuclear lysate forDNMT activity analysis from cells?

Hello everyone. I want to do DNMT activity analysis by using prostate cancer cells. I want to know whether we can store the nuclear lysate for 2-3 days in -20 degree before performing activity...

05 June 2018 6,568 7 View

Do we need to quantify bisulfite treated DNA for using it as a template for methylation specific PCR?

Hello everyone I have bisulphite converted my DNA using kit from Zymo research now I have to do methylation specific PCR. I have to check effect of my drug on methylation status of diff. genes...

10 November 2017 8,380 4 View

Can we use autoclaved DEPC milliQ water for bisulpgite conversion and methylation specific pcr?

I am going to do ms pcr but I have doubt on water milliQ water because previously RT pcr result was not coming by using that water. So I borrowed water from another department but that water was...

09 October 2017 4,011 4 View

RBC contamination in WBC pellet during DNA isolation from blood?

Hello everyone, I isolated DNA from freshly taken Human blood but during processing I was getting RBC contamination in pellet .I washed with RBC lysis buffer 6-7 times followed by...

09 October 2017 5,451 3 View

Can we use 3-4 month old DNA for methylation studies?

Hello, I have 3- 4 month old DNA stored at - 2O degree. Can I use thisDna for bisulphite treatment followed by methylation specific pcr? Thank you

31 December 2016 5,366 5 View

DNMT activity assay?

Hello, I want to do radioactivity based DNMT activity assay. Can somebody upload the whole prtocol for this? Thank you

06 July 2016 2,258 5 View

Has anyone done RT PCR for Cyclin D2 , p21 and p27 in DU145 cells ?

I have done RT -PCR for Cyclin D2, p21 and p27 in du145 , but did not get any band . in p21 , there was v. faint band but when I repeated the same , I did not get any band for p27. does these 3...

03 April 2016 928 1 View

How to freeze LNCaP cells?

What is the best way to freeze LNCaP cells? ATCC recommends complete medium plus 5% DMSO. some people use 10% DMSO, 20% FBS and rest complete medium. Some use 90% FBS and 10% DMSO. Please tell me...

31 December 2015 2,459 3 View

Why do we need to do the conditioning of vessels with media or PBs in rotary cell culture system?

we purchased RCCS from synthecon Inc. In users guide it is mentioned to prepare vessels by rinsing with media or PBS for 5 mins to overnight. I want to know the purpose of doing this...

31 December 2015 4,483 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View