Do all the mRNAs of pox virus are polyadenylated at 3' end?

Hello everyone, In eukaryotic cells, all the mRNAS are polyadenylated at 3' end. I want to ask whether this is the same case for pox viruses also. Do all their mRNAs are polyadenylated at 3'end...

04 May 2019 3,219 2 View

Can we use freezed nuclear lysate forDNMT activity analysis from cells?

Hello everyone. I want to do DNMT activity analysis by using prostate cancer cells. I want to know whether we can store the nuclear lysate for 2-3 days in -20 degree before performing activity...

05 June 2018 6,439 7 View

Do we need to quantify bisulfite treated DNA for using it as a template for methylation specific PCR?

Hello everyone I have bisulphite converted my DNA using kit from Zymo research now I have to do methylation specific PCR. I have to check effect of my drug on methylation status of diff. genes...

10 November 2017 8,291 4 View

Can we use autoclaved DEPC milliQ water for bisulpgite conversion and methylation specific pcr?

I am going to do ms pcr but I have doubt on water milliQ water because previously RT pcr result was not coming by using that water. So I borrowed water from another department but that water was...

09 October 2017 3,899 4 View

RBC contamination in WBC pellet during DNA isolation from blood?

Hello everyone, I isolated DNA from freshly taken Human blood but during processing I was getting RBC contamination in pellet .I washed with RBC lysis buffer 6-7 times followed by...

09 October 2017 5,351 3 View

Can we use 3-4 month old DNA for methylation studies?

31 December 2016 5,271 5 View

DNMT activity assay?

06 July 2016 2,163 5 View

Has anyone done RT PCR for Cyclin D2 , p21 and p27 in DU145 cells ?

I have done RT -PCR for Cyclin D2, p21 and p27 in du145 , but did not get any band . in p21 , there was v. faint band but when I repeated the same , I did not get any band for p27. does these 3...

03 April 2016 839 1 View

Why do we need to do the conditioning of vessels with media or PBs in rotary cell culture system?

31 December 2015 4,391 2 View

How to freeze LNCaP cells?

What is the best way to freeze LNCaP cells? ATCC recommends complete medium plus 5% DMSO. some people use 10% DMSO, 20% FBS and rest complete medium. Some use 90% FBS and 10% DMSO. Please tell me...

31 December 2015 2,323 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View