I want to use Glutathione-S-transferase (GST) + monochlorobimane based assay kit that specifically detects GSH in tissue samples such as brain, kidney etc.
This is 96-well plate based assay. I want to make a Master Mix of all reagents (buffer + GST enzyme+substrate) and add to the samples rather that add them individually.
However, the Sigma-Aldrich kit (CS1020) neither recommends nor rejects it.
Kindly guide if anybody has used this kit whether making a master mix is OK?