Can we get protein from sds page in micromolar or somewhat higher quantities?
I have some literature reports which suggest that the functional groups present on the surface of the activated carbon get depleted. Domingues RR, Trugilho PF, Silva CA, et al. Properties of...
10 November 2018 2,911 6 View
It is also said to do the F-test after non-linear regression of the Langmuir model and Freundlich model for deciding the better fitted model between the two. Why so?
03 April 2018 3,454 0 View
We have some data. We have one model convertible into linear and non-linear form. I want to ask if we model the same data after conversion, results for both linear and non-linear forms for the...
01 February 2018 9,242 3 View
Please help me in getting rid of sericin gelation? Everytime I am extracting sericin, it is getting gelated in one way or the other. I don't know why it is happening but it is happening. It would...
09 October 2015 8,582 2 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...
03 March 2021 8,237 3 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...
25 February 2021 7,698 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...
22 February 2021 5,324 3 View