Yes you can

Yes i do I use thick and thin blood smear film technique and

microscopy

Parasites may be found in a splenic aspirate, liver biopsy or bone marrow biopsy.  

.. They are approximately 2-5µm in size, oval and are frequently seen within the cytoplasm of the macrophage. The amastigotes possess a nucleus and a rod - shaped kinetoplast within the cytoplasm. In many samples a very small number of parasites are present.   

Thanks a lot for your valuable answers 

I endorse dear Juan Otaso's answer;Thanks

Thank you.

Hi

Yes it is possible.

I agree with Juan Otaso: the amastigotes may be found in a splenic aspirate, liver biopsy or bone marrow biopsy with high sensitivity. But in my opinion, the chances of finding the amastigotes in a thick or thin blood film, using microscopy, are too small. Also in a thick blood film, any amastigotes might be too difficult to identify correctly. To detect visceral leishmaniasis in blood, molecular techniques might be more appropriate.

Thanks for your answer Idz. I agree with you because I tried to identify the parasite in the blood but it was not effective.

One more question. If the parasite existence not that much clear in the blood. how is the transmission between humans. I mean from where the sand fly can take the amastigotes if it is not available in the blood?

The amastigotes might be available in the blood, but the sensitivity of a blood film is just too low to detect it there. The sandfly needs just one amastigote to infect itself. If you look at malaria for example, the sensitivity of a blood film with expert microscopy is about 50 parasites per microliter. 

Always bear in mind that the procedures used in the diagnosis of Cutaneous leishmaniasis (LTA) depend largely on the purpose and infrastructure of the laboratory in which we work. Furthermore, it is known that, due to clinical polymorphism LTA, obtaining samples vary methods demonstration and isolation of parasites. Laboratory tests are grouped into direct or indirect parasitological or immunological.

Direct methods or parasitological

In parasitological diagnosis there are two alternatives. The first is to demonstrate that the patient is harboring leishmania by displaying, smear or histopathology of amastigotes in infected tissues. The second option is to attempt the direct isolation of promastigotes in vitro of suspicious lesions crops.

Another method used is inoculating laboratory animals (golden hamsters) and isogenic and non-isogenic mice, from which can be isolated and characterize Leishmania through PCR (polymerase chain reaction), monoclonal antibodies and / or isoenzyme electrophoresis.

In mucosal lesions

In connection with single or multiple mucosal forms, methods generally used are biopsy using special nippers (cutting punch biopsy) and frotises biopsies. L. (V) brasiliensis is difficult to diagnose in the mucosal granulomas. Positivida with a percentage of 50% in only 70 %% mucosal lesions and multiple lesions, 27.4% in smears of biopsies and 16% in the histopathologic

It is uncommon to find amastigotes of Leishmania in blood. Some studies reported that in immunocompromised HIV infection.

Hi Bodour Alassil

In case of VL, the best method to get the amastogote (parasitic stage) in macrophages is biopsy of liver and bone marrow. Splenic aspirates also useful in detecting the parasite.

In blood, parasite may be detected but the chances are low as the VL is a tissue infection but establishes through blood stream, therefore it is possible.

The more chances are

1. in the initial stage of infection and

2. when the infection has established and host is diseased

Thank you

Good luck