Hello. I am having troubles with serum samples. I know that they are positive for Leishmania but when i do the PCR, most of my samples are negative. So, any ideas to have a better outcome? Maybe some extra step.... I use a MagMax kit for extraction, but i can also use Promega and Nzytech. Thank you
The serum is more suitable for serological analyses (antibody detection) not for PCR. For parasite DNA detection, It's better to start with clinical samples: tissues, whole blood samples, or Blood Buffy Coat.
Article Real-time PCR applications for diagnosis of leishmaniasis
Yes, i know. I already did in other biological matrices, like blood and bone marrow. But, we wanted to bring up some challenge and try to do PCR in serum samples. We have some ideas, but i just wanted to see if there was a different perspective.