I need any one of these strains for my research.
I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...
27 February 2021 4,703 1 View
Here I am facing this problem. I don't find any effect of this type of parameters. I am a beginner user of COMSOL.
23 February 2021 9,086 3 View
Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...
23 February 2021 1,362 5 View
I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...
23 February 2021 4,050 1 View
I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?
22 February 2021 215 2 View
As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?
19 February 2021 5,509 3 View
I have remotely sensed data (image) that I want to use in the Neuro-Fuzzy Designer apps of MATLAB. Is it possible to use an image file in the Neuro-Fuzzy Designer app or only numeric data is...
17 February 2021 1,205 5 View
I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...
09 February 2021 9,012 3 View
I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...
04 February 2021 6,580 2 View
The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...
31 January 2021 729 6 View
I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...
15 August 2020 1,898 2 View
i need an easiest technique for Fluorescent stain used with Fluorescent Microscope .
14 July 2020 3,551 3 View
Hi, In my work, I evaluate the role autophagy play in Leishmania infection. Using confocal we obtained images of human macrophages infected with Leishmania labelled with LC3. Now, I would like...
08 September 2019 6,821 3 View
I have done Romanowsky stain on macrophages infected with different Leishmania clinical isolates. Now I want to count the infected cells to calculate the endocytic rate (% infected macrophages x...
08 September 2019 7,791 1 View
Hello all, I am working with mouse organs infected with Trypanosoma cruzi parasites. When animals are killed they are in the chronic stage of the disease, and their organs are extracted in...
25 August 2019 5,193 2 View
07 August 2019 8,894 1 View
While working with Leishmania donovani , I got to know that it is grown in non-vented culture flasks with M199 media (as preferred in my lab) and kept in BOD incubators. Some researchers also...
06 July 2019 9,014 1 View
The literature reveals array of assay including assay using tetrazolium dyes, using mix of sybr green in Triton, counting using cytometer, etc. I would appreciate if the expert working in said...
04 May 2019 3,148 3 View
Can anyone recommend a good PCR primer for Trypanosoma genus? That can target all species, or at least most of the Trypanosoma species? Thank you
01 February 2019 1,282 2 View
Please check the picture quoted from a textbook. Iam interested how the nucleus is stained red by Giemsa stain ?! It has to be purple in colour
31 December 2018 5,246 3 View
