I need any one of these strains for my research.
kindly reply me. Thanking you in advance.
05 August 2024 7,726 4 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,282 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
What is the future scope of acoustic emission?
17 July 2024 1,509 1 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
14 July 2024 950 2 View
I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.
14 July 2024 777 0 View
I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?
12 July 2024 3,745 2 View
In the expression for solid solution strengthening model that has been widely used in the literature use a linear form consisting of strength coefficient, and concentration of elements in wt.%....
11 July 2024 4,476 3 View
I am trying to measure the plasmon resonance angle of gold film and pure water using the Kretschmann configuration and a 633nm laser. Without flowing water over the gold, I can detect a clear...
10 July 2024 4,719 3 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
my question about the sequence analysis I did whole genome sequence for a leishmania parasite I got the sequence of rDNA in stead of GDNA can anyone ?
30 May 2024 8,671 0 View
Hi, I am trying to express Trypanothione synthetase a protein from Leishmania, cloned in pET28a vector and transformed in E.coli. BL21 (DE3). The size of the protein is 74kDa. Initially, I...
21 May 2024 4,701 5 View
I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...
12 May 2024 1,045 4 View
Hello. I am having troubles with serum samples. I know that they are positive for Leishmania but when i do the PCR, most of my samples are negative. So, any ideas to have a better outcome? Maybe...
10 March 2024 9,384 2 View
Which medium it's the best for growing Leishmania mexicana?
25 November 2023 7,675 3 View
Not been able to find any article on this. Help me to find out.
09 November 2023 1,540 1 View
I'm facing challenges with the purity of plasmid DNA extracted from Trypanosoma brucei for PCR applications. Despite using a commercial DNA extraction kit, I suspect the presence of contaminants...
20 October 2023 829 3 View
I am currently in the planning stages of conducting experiments involving the labeling of Leishmania parasites using CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester...
18 September 2023 8,157 1 View
I plan to tag a gene in Leishmania donovani, and it would be a big help if anyone could help me decide the tagging position, whether it could be 5' or 3'. I also plan to do the DiCre Cas9 edition...
28 June 2023 4,441 0 View
