Where to get non-transformed non-tumorigenic human hepatocytes?

07 August 2019 1,785 2 View

What primer (Human or mouse) I should use for qPCR on xenograft tumor samples?

Recently I did qPCR using mice xenograft tumor samples. I got very good amplification of internal loading control GAPDH but not my target gene using human Taqman probes. (although I am not sure at...

09 October 2018 7,964 4 View

Reasons of tumor ulceration in MDA-MB-231 xenografts established in NSG mice?

Recently, I implanted breast MDA-MB-231 tumor cells in NSG mice. Surprisingly, some mice showed ulceration in their tumors at tumor size around 200-300 mm3 much below the humane endpoint. I never...

08 September 2018 6,697 6 View

Best equal loading control protein for different cell types on same western blot membrane?

I recently did the baseline levels of some proteins in different tumor cell lines and used b-actin as equal loading control. I am struggling to get equal b-actin in different cell types. I have...

06 July 2018 8,697 8 View

How to quantitatively measure the relative levels of two or more proteins within a cell line?

Is there a way to quantitatively measure the relative levels of two or more proteins in a single cell line? In the linked paper the authors have used Quantitative IR-fluorescence western to do...

10 November 2017 4,580 7 View

How to avoid IgG bands in Co-immunoprecipitation experiment?

I am getting IgG heavy and light chain bands at 55 and 25 kDa, respectively, in my Co-IP experiment. These bands are obscuring my proteins of interest which are close to these molecular weights....

08 September 2017 1,148 11 View

Normal platelet count in human versus mice?

08 September 2017 1,570 9 View

How to record the passage number of suspension cells?

Is it necessary to record the passage number of suspension cells, for example AML and ALL leukemic cells? If so, how one should record it since like adherent cultures we don't really split them...

06 July 2017 9,279 6 View

Why some protein inhibitors increase the levels of target protein and still known as their inhibitors?

01 February 2017 7,863 5 View

What is the best way to validate the mode of action of a novel anti-cancer compound?

While validating the mode of action of a novel anti-cancer compound, we have options of using small interference RNA or pharmacological inhibitors of a particular signaling molecule. In several...

31 December 2015 1,182 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View