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Questions related from Sajid Khan
I am looking for non-transformed non-tumorigenic human hepatocytes that can be maintained in culture. I found one in ATCC i.e THLE-2 (ATCC® CRL-2706™), but this is a transformed cell line,...
08 August 2019 1,849 2 View
Recently I did qPCR using mice xenograft tumor samples. I got very good amplification of internal loading control GAPDH but not my target gene using human Taqman probes. (although I am not sure at...
10 October 2018 8,025 4 View
Recently, I implanted breast MDA-MB-231 tumor cells in NSG mice. Surprisingly, some mice showed ulceration in their tumors at tumor size around 200-300 mm3 much below the humane endpoint. I never...
09 September 2018 6,794 6 View
I recently did the baseline levels of some proteins in different tumor cell lines and used b-actin as equal loading control. I am struggling to get equal b-actin in different cell types. I have...
07 July 2018 8,759 8 View
Is there a way to quantitatively measure the relative levels of two or more proteins in a single cell line? In the linked paper the authors have used Quantitative IR-fluorescence western to do...
11 November 2017 4,638 7 View
I am getting IgG heavy and light chain bands at 55 and 25 kDa, respectively, in my Co-IP experiment. These bands are obscuring my proteins of interest which are close to these molecular weights....
09 September 2017 1,305 11 View
The normal platelet count in humans is 150,000-400,000 per uL of blood. Can anybody tell me what is the normal platelet cell count in mice? Does mice suppose to have higher platelet count/uL of...
09 September 2017 1,639 9 View
Is it necessary to record the passage number of suspension cells, for example AML and ALL leukemic cells? If so, how one should record it since like adherent cultures we don't really split them...
07 July 2017 9,341 6 View
In an experimental condition (e.g. high concentration of cytotoxic drug) there occurs more than 90% cell death, then is it possible to get equal b-actin (or other loading control protein) as in...
06 June 2017 7,647 0 View
I am wondering why some protein inhibitors increase the levels of target protein and still known as their inhibitors? For example MCL1 inhibitor S63845 increase the levels of MCL1 in tumor cells...
02 February 2017 7,954 5 View
While validating the mode of action of a novel anti-cancer compound, we have options of using small interference RNA or pharmacological inhibitors of a particular signaling molecule. In several...
01 January 2016 1,241 5 View
