What primer (Human or mouse) I should use for qPCR on xenograft tumor samples?

Recently I did qPCR using mice xenograft tumor samples. I got very good amplification of internal loading control GAPDH but not my target gene using human Taqman probes. (although I am not sure at...

09 October 2018 7,964 4 View

Reasons of tumor ulceration in MDA-MB-231 xenografts established in NSG mice?

Recently, I implanted breast MDA-MB-231 tumor cells in NSG mice. Surprisingly, some mice showed ulceration in their tumors at tumor size around 200-300 mm3 much below the humane endpoint. I never...

08 September 2018 6,697 6 View

Best equal loading control protein for different cell types on same western blot membrane?

I recently did the baseline levels of some proteins in different tumor cell lines and used b-actin as equal loading control. I am struggling to get equal b-actin in different cell types. I have...

06 July 2018 8,697 8 View

How to quantitatively measure the relative levels of two or more proteins within a cell line?

Is there a way to quantitatively measure the relative levels of two or more proteins in a single cell line? In the linked paper the authors have used Quantitative IR-fluorescence western to do...

10 November 2017 4,580 7 View

How to avoid IgG bands in Co-immunoprecipitation experiment?

I am getting IgG heavy and light chain bands at 55 and 25 kDa, respectively, in my Co-IP experiment. These bands are obscuring my proteins of interest which are close to these molecular weights....

08 September 2017 1,148 11 View

Normal platelet count in human versus mice?

08 September 2017 1,570 9 View

How to record the passage number of suspension cells?

Is it necessary to record the passage number of suspension cells, for example AML and ALL leukemic cells? If so, how one should record it since like adherent cultures we don't really split them...

06 July 2017 9,279 6 View

Can we detect b-actin in the cell lysates if cell death is more than 90%?

05 June 2017 7,556 0 View

Why some protein inhibitors increase the levels of target protein and still known as their inhibitors?

01 February 2017 7,863 5 View

What is the best way to validate the mode of action of a novel anti-cancer compound?

While validating the mode of action of a novel anti-cancer compound, we have options of using small interference RNA or pharmacological inhibitors of a particular signaling molecule. In several...

31 December 2015 1,182 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View