Serial dilution and counting them after 15-24 hours could helps you.

Depending the fungi you are working on, you can find appropriate culture media which limit the growth area of fungus colonies on the agar surface .Serial dilution and counting before complete growth of colonies may be useful.

Dear Das,

Please go through the attached pdf file, to be specific Page 6.

Please observe the link.

With regards,

Ankur J Saikia

Article Enumeration of colony forming units of Trichoderma in formul...

Dear Saurav,

I agree with the opinion that it's possible to enumerate CFU by serial dilution method and further usage of formulas as it accepted for bacteria, at least for soil anamorphic micromycetes. Extremely fast growing fungi such as Trichoderma and Mucorales can be a problem but for majority of anamorphic Ascomycetes the method works.  I've used non-selective media such as Czapek and Malt agar and 10-4 dilution. It is also helpful to store Petri dishes in bottom-up position to prevent spores of abundantly sporulating species  to originate new additional colonies during the experiment.

Best regards,

Elena

@ Elena

It also works nicely for fast growing Mucorales. In that case you need to look at the plates as long as the colonies are very small.

For many Mucor-like fungi, it is possible to keep the colonies very compact by plating them on media at pH=3.0. This pH can pe reached by buffering the normal medium components (don't use malt extract or similar ingredients with high buffer capacity  for this purpose!) with citric acid/citrate. Autoclave the medium components separate from the agar (in one half of the water)  and mix after cooling to 65 degrees. This works nicely for Absidia glauca and others.

@ Johannes Wöstemeyer 

Dear Dr Wöstemeyer,

Thank you very much for helpful instructions,

Elena

.... only a small addition ... just in case. :-)

@Elena  and @Johannes

Dear Voronina and Dr. Wöstemeyer

Thank you very much for all the helpful instructions. Much appreciated.

Saurav

We can calculate fungus by CFU after sporulation.....

@Sambhaji Chavan .. How ?? it will be helpful if you can elaborate a bit ??

I think it seems like that... after sporulation, collect spores and diluted them serially. Then spread the aliquot similar to spread plating technique. Each spore will grow as a single colony and one can estimate the number of spores in per ml of the solution. 

113

I think there are no any media you can estimate the spore-like bacteria, but only two technique 1. hemocytometer, 2. diluted with spread plating

Thank you