Like other aminoglycosides, paromomycin has no ultraviolet (UV) absorbing chromophores, and therefore the derivatization of the amino groups is required for high-performance liquid chromatography (HPLC) analyses with UV detection. A HPLC method with fluorescence detection is also developed with post-column derivatization with ortho-phthalaldehyde (OPA) and 2-mercaptoethanol. Pre-column labeling with OPA is not used because of the low stability .
of the derivative. Because of the thermal instability of aminoglycosides, the analysis of aminoglycosides by GC or gas-chromatography/mass spectrometry (GC/MS) is difficult . The analysis of paromomycin by evaporative light-scattering detection (ELSD) has not been reported previously. ELSD has been applied as a universal detection method .