Hello everybody, I work with iPSC, differentiating them into neurons.
At some point some of my stem cell popultion seem to have differentiated into a mesodermal type of cells. Is there a way of "reprogramming" those mesodermal cells in order to get them back to the stem cell status so then I can differentiate them into neurons (ectodermal pathway)?
Thanks in advance
Yohei Kono
It is well known that iPSCs preserve the epigenetic memory of the derived tissue. Are your iPSCs possibly mesoderm-derived? If you want to differentiate them into neuron-only, you should establish iPSCs from neurons, but if that is difficult, I think you have to passage them through more and more generations to erase the epigenetic memory or proactively modify the epigenetic.
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