How to calculate Leaf Water Conten Index (LWCI)?

I want to calculate Leaf Water Conten Index (LWCI) using Landsat or Sentinel data. I found several usefull formulas that use NIR and SWIR bands. But to estimate LWCI I have to have NIR and SWIR...

16 March 2020 9,322 4 View

Real Time PCR Analysis and Normalization?

If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...

18 December 2018 6,267 2 View

Gene Expression Analysis using R?

Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?

13 December 2018 5,140 5 View

Monoclonal antibody purity?

If monoclonal antibody would be obtained from ascites fluid during hybridoma expression in vivo -> protein A/G-sepharose purification, how much non-specific AB contamination and non-target...

04 December 2018 6,582 3 View

Does the plasmonic near field spread on the distance sufficient for the resonance light absortion (scattering)?

Cross section of light absorption and light scattering for noble metal (silver, gold) nanoparticle is higher than its geometrical cross section in case of plasmon resonance. It can be...

30 October 2018 5,235 3 View

Degenerate Primers to ITS Region (16S-23S rRNA) for NGS?

F: 50 -CTKTTGGGYYCCCKGRYYG-30 Rev: 50-CGCGTCCACTMTCCAGTTCTC-30 These are primers for NGS sequencing of bifidobacteria from a publication. If you substitute, for example K, with G or T then...

09 April 2018 6,351 3 View

How to obtain a precipitated powder of Poly-Amic Acid based on aromatic diamine and aromatic dianhydride?

How to obtain a precipitated powder of Poly-Amic Acid (PAA) based on aromatic diamine and aromatic dianhydride? It is well known that for several Aromatic Polyimides (PIs) in the course of...

22 December 2017 9,399 3 View

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film ?

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film

17 August 2017 1,331 1 View

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know?

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know? I know about stegoDB but can't find download link. Thanks

10 April 2017 8,150 9 View

Is that C. jejuni?

Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....

26 March 2017 3,470 12 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View