Will it inhibit PCR?
Thank you
We ran short of TE, but we only have TBE and TAE.Has anyone tried storing plasmid DNA with TBE or TAE and would they inhibit downstream applications such as transformation?Thanks so much for...
02 March 2016 2,588 8 View
Send me a message by clicking my profile > info > Send message. I am looking for a BL21 (DE3) e.coli train with a pET plasmid for non-specific purposes. No tag is needed, it just needs to...
07 August 2015 6,312 0 View
Was it something like this? would something like this work?1. Extract DNA from sample2. Restriction digest sample DNA and vector backbone DNA3. Deactivate enzyme4. Ligate and transform Is this how...
07 August 2015 1,779 5 View
Or could someone kindly point me in the right direction with a link?Would be greatly appreciated..
07 August 2015 687 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
