Damien, TE buffer is 10 mM Tris (pH 8.0, HCl) and 1 mM EDTA. You can make it in 1 Minute if you have the stock solutions. If you don't, just store your DNA frozen in sterile water. This is very trivial, indeed :)

I think that it can not use TBE or TAE to store DNA, there are some other component in them,  such as ion,so it will influence the ion concentration for PCR or other experiment, in our laboratory,we sometimes use ddH2O.

Damien, TE buffer is 10 mM Tris (pH 8.0, HCl) and 1 mM EDTA. You can make it in 1 Minute if you have the stock solutions. If you don't, just store your DNA frozen in sterile water. This is very trivial, indeed :)

TAE has acetate which is enzyme inhibitor usually we use isolated DNA for PCR, Restriction digestion ETC where we use enzyme so if acetate is present it will inhibit some processes don't exactly know about the down stream processing

Hello Damien,

Unlike acetic acid in TAE, borate in TBE is strong inhibitor of many enzymes and therefore DNA intended to be used for cloning purposes etc., is best run in TAE buffer. The final concentration of Tris, acetic acid, and EDTA in TAE is 40, 20, and 1 mM respectively. DNA is commonly stored in TE composed of 10 mM Tris and 1 mM EDTA, pH 8.0. Alternatively, 5-10 mM Tris-Cl buffer (pH 8.0) can also be used to store DNA. The purpose of EDTA (0.1-1 mM) is to chelate metal ions so that the DNA is less prone to DNAse -mediated degradation. However, it is not mandatory to include EDTA in DNA storage buffers. For instance, most plasmid DNA extraction/purification kits use 5 mM Tris-Cl, pH 8.5 buffer as DNA elution/storage buffer.

Now can TAE be used to store DNA? Well, why not prepare TE as mentioned by Dr. Andriy for it is so simple to prepare. If you still want to store the DNA in TAE, I believe it is possible. We do lots of cloning and at times, cut our DNA fragments from agarose/TAE gels and store DNA-containing agarose chunks at -20C until use. We have stored our DNA fragments sometimes for several weeks and recovered them intact from agarose/TAE without any problem. This suggests that DNA is fine in TAE. Simply dilute the 1X TAE 8 fold so that final concentration of Tris, acetic acid, and EDTA is 5, 2.5, and 0.125 mM respectively and use this diluted buffer for DNA storage.

That said, storing in either TE or Tris-Cl buffers as suggested above would be ideal.

The recommended method for  long storing DNA is 10mM Tris (ph 8.5) . This method can be utilized for both column and reagent based DNA extraction but TE will not work out for column based method, why because TE will not bind to the column.

ideally pH =8 or slightly higher should be used. any buffer is OK. TE is the most popular

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

TAB buffer is a general restriction enzyme buffer. Because it works pretty well with almost all restriction enzymes (including SmaI & other KCL-prefering enzymes), it makes double & triple digests particularly easy. This is essentially the buffer recommended by Maniatis for use in blunting 3' or 5' overhanging ends with T4 DNA polymerase.