I ordered some bacteria from DSMZ. One of them is freeze dried ampule and the other is actively growing culture. I would like to learn, should I use the bacteria strains as soon as I receive them or I can store them in optimum storage conditions in a few days?
Because I have to inoculate the bacteria in different days and in different product. Are these freeze dried ampoules and actively growing culture disposable or I can sustain the culture during a few days?
Dear Dr. Goris,
many thanks for your contribution. I would like to be sure of about actively growing culture. Upon receipt should I prepare the pre or stock culture from actively growing culture? Or it s already a preculture in agar slants or in broth?
Thanks,
Dear Dr. Karen
many thanks for the contrubition. So you mean I must prepare subculture from actively growing culture as soon as I recieve. But I m wondering about this, how I know in which time I will have how much cfu/ml cells number. Dont I need to define my cell number in the culture with OD or plate count? And after being sure the cell number in the culture I must inoculate the culture to fish sample? I m confused, could you shed light please on this subject if you dont mind?
Bests,
Ilknur
Hi Karen,
They are Morganella psychrotolerans and Photobacterium phosphoreum
Dr. Goris,
No problem, I m still searching about this subject. In addition I m waiting to recieve my strains both freeze dried ampoules and actively growing culture. I could conduct the freeze dried ampoules easily since I m used. But I m not sure to be success on actively growing culture. It will be the first time to work with this.
by the way, many thanks for your contributions.
I have received my both cultures today and I preperad the subcultures. I will see, hopefully I can see the colonies and growing in medias.
Thanks a lot
Hi Ilknur,
May I know how's your culture work? I just received my strains today (actively growing). But, I would like to keep them for a couple of days before I subculture them. I am not sure if that is okay? Thank you very much.
Best wishes,
Chin Yik
Dear Chin,
As I know you should subculture your strains as soon as recieve them. Or you can ask where you get them.
Bests
ilknur
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