If you are interested in RNAseq, why do you care about 18S or 28S bands?

Also, Attaching figure does not mean attaching whole pptx file and let others do the job of opening file and interpret image for you. Please attach the image and not presentation.

Thank you for your answer.

In this study, I will do RNA-seq analysis, and in the other study, I will synthesize cDNA. There will be problem with cDNA production. The integrity of the RNA is important for the production of full-length cDNA. So i'm interested in rRNA bands.

Regards

The 28S and 16S rRNA band intensities on the gel are clearly indicative of RNA degradation.

The 28S and 18S rRNA band intensities on the gel are clearly indicative of RNA degradation.

Thank you for your answer.

I take a very precautions to avoid RNase contamination. Is the degradation of the bands physical or enzymatic? Is it possible to understand this? How can I prevent degradation?

Best regards

Dear Ilknur Külahlıoğlu Çeğil

Can I ask how was your total RNA concentration?

For RNA-seq and more important for cDNA synthesis, the concentration can be a little more critical.

Dear Shera Haghir thank you for your answer.

According to the last few protocols, the RNA concentration ranges from 300 to 1200 ng/ul. When loading the RNA samples into the gel, I dilute to 250 ng/µl and load 2 µl (500 ng) to each well.

Regards