You can use nutrient broth add 6,9,11,17 salt divide on tubes count. 10ml and 0.1bacteria test are add to tubes after incubation at 35c for 24-48 hours .The tubes determined by spectrophotometer at 600 n m than standard sampls
It is possible by using spectrophotometric method, how ever the dead cell may interfere in the reading. But it is better to use Zobel marine agar and serial dilution followed by spread plate method. Before performing this we should know wether we are going to isolate/enumerate mild, moderate or extreme halophile. Accordingly we will vary the salt concentration in the medium.