What is the impact of AGL1 Agrobacterium strains on regenerated plant phenotypes? AGL1 is hypervirulent. I also heard that some plants regenerated from calli are erratic. Are there any papers claimimg so? If you could find me a paper stating the same.
Hi,
I would recommend you those articles. The efficacy of agrobacterium AGL1 depends on the relationship with the host and the tissue to infect. I have to say that however the AGL1 gave sufficient results to be used but I have used for ATTA assays, and have experienced better results with other strains in N. benthamiana.
Article Acetosyringone treatment duration affects large T-DNA molecu...
https://pdfs.semanticscholar.org/5e8c/7c7615745d2f2a67b58c58d4613f9f3f83bb.pdfArticle Evaluation of Four Agrobacterium Tumefaciencs Strains for th...
Best regards
A lot of researchers have used AGL1 for genetic transformation on different plant species. Plants regenerated from calli with erratic phenotype are not necessary due to the Agrobacterium per se. The erratic phenotype or regenerants could be raised from tissue culturing practicing, or due to somaclonal variation.
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