Also, I want to ask if I worked with agrobacterium I need to design a special plasmid for my fungus transformation or I can use the plasmid used with other species?
Yuan-Yeu Yau
Most likely you need to design a special plasmid for your research need. You need to use a promoter which can drive and make your gene-of-interest express in your fungus.You might need to clone a specific gene into the plasmid for your research purpose.
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Aditi Nadkarni
Check this out: http://www.currentprotocols.com/WileyCDA/CPUnit/refId-mc03d02.html
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