Ayat Ibrahiem Esmaeel Al-Laaeiby

14 Questions 37 Answers 0 Followers

Questions related from Ayat Ibrahiem Esmaeel Al-Laaeiby

Three-dimensional Gel Electrophoresis?

Does anybody have information regarding using 3D electrophoresis in the studying gene expression? Does this method could analyse the expression of all genes in the organism or it target specific...

28 November 2018 8,190 3 View

Does anybody have an idea regarding minus value in statistic analysis?

I got some minus value in the my results, I repeated the experiment three time, and get same values. Does anybody could advice me that how can I treated them statistically? Thank you

19 May 2017 5,482 10 View

Does anybody used the formula below?

I want to count killing percentage by using MTT assay. I incubated spores with macrophage in time course and used MTT dye for overnight, dye concentration 0.1 mg/ml. My question is that I want to...

16 August 2016 7,412 2 View

How can I select suitable label and dye in flow cytometer?

Dear all, I am trying to count alive spore from dead one after incubation with macrophage by using flow cytometer, Does anybody has an idea, how can I select suitable label to my spores and...

17 July 2016 7,799 5 View

Does anybody have macrophage lysis protocol?

I want to lysate macrophage after co-incubation. Does anybody share protocol to lysis macrophage? I know, It could be lysis by dipping in the liquid nitrogen but I want protocol does not affect...

30 May 2016 949 5 View

Does anybody comfirmed the TOXA:GFP transformants with southern blot?

I transformed my strain with TOXA:GFP and trying to confirm the insertion by using southern blot technique. I can see the fluorescent under the microscope but it does not work with ELISA or...

28 April 2016 2,963 2 View

Any advice on sensitivity to an antifungal drug test?

I read papers that they used two methods to study antifungal sensitivity. My question is why they use more than one way for, example E-test and broth method? it is important to use two ways? I...

24 September 2015 6,753 7 View

Integration mechanism of filamentous fungi?

Does anyone could explain the mechanism of DNA insert after cloning to a filamentous fungus and what is the fate of plasmid (which carry the insert)? Regards

16 September 2015 9,520 4 View

How can I prepare a spore suspension 10^9 or 10^8 in 1ml?

I account my spore number its 3.6*10^6 spores/ml. Can anyone help me to reach this concentration? Regards

16 October 2014 906 4 View

How can I decipher between linear and circular plasmid on gel electrophoresis?

I used two restriction enzymes to cut my PCB1532 plasmid but I do not know when I run on gel how can I distinguish if my enzyme are not work properly. I cut this vector to use on fungal...

01 July 2014 8,346 14 View

Does anyone have experience cloning 10kb fragment?

I want to clone 10kb fragment to complete the complementation on fungi, could someone advise me how can I clone long fragment because I did before cloning 4kb fragment with classic methods. I do...

24 June 2014 6,961 3 View

What is the difference between using southern blot and gene complementation after a knockout gene?

Why did we use complementation inspite of how we examine how many copies of the gene mutated by southern blot?

04 May 2014 884 1 View

When I developed my southern blot, I found a black dot that covered and hid my results. What should I do to solve this problem?

DIG southern blot

08 April 2014 1,453 12 View

When selecting suitable enzyme for Southern Blot, should it be cut on ORF, LF, RF in wild type and hygromycin, RF, LF in mutant?

I am confused about selecting of enzyme for a Southern Blot and where exactly to cut it. I am working with knock out and used hygromycin selectable marker.

27 February 2014 571 1 View