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Questions related from Ayat Ibrahiem Esmaeel Al-Laaeiby
Does anybody have information regarding using 3D electrophoresis in the studying gene expression? Does this method could analyse the expression of all genes in the organism or it target specific...
28 November 2018 8,257 3 View
I got some minus value in the my results, I repeated the experiment three time, and get same values. Does anybody could advice me that how can I treated them statistically? Thank you
19 May 2017 5,551 10 View
I want to count killing percentage by using MTT assay. I incubated spores with macrophage in time course and used MTT dye for overnight, dye concentration 0.1 mg/ml. My question is that I want to...
16 August 2016 7,459 2 View
Dear all, I am trying to count alive spore from dead one after incubation with macrophage by using flow cytometer, Does anybody has an idea, how can I select suitable label to my spores and...
17 July 2016 7,842 5 View
I want to lysate macrophage after co-incubation. Does anybody share protocol to lysis macrophage? I know, It could be lysis by dipping in the liquid nitrogen but I want protocol does not affect...
30 May 2016 993 5 View
I transformed my strain with TOXA:GFP and trying to confirm the insertion by using southern blot technique. I can see the fluorescent under the microscope but it does not work with ELISA or...
28 April 2016 3,032 2 View
I read papers that they used two methods to study antifungal sensitivity. My question is why they use more than one way for, example E-test and broth method? it is important to use two ways? I...
24 September 2015 6,821 7 View
Does anyone could explain the mechanism of DNA insert after cloning to a filamentous fungus and what is the fate of plasmid (which carry the insert)? Regards
16 September 2015 9,570 4 View
I have 5 isolate(wild type, 3mutants and 1 complement) and I want to compare between wild type and them. My question is how can I get t.test and p.value? I created each isolate with control...
20 June 2015 8,653 2 View
I am looking for information regarding melanin biosynthesis in an evolutionary context. I searched a lots on books, papers, web sites but unfortunately, I cannot catch anything. Kindly, if someone...
03 June 2015 9,058 5 View
I account my spore number its 3.6*10^6 spores/ml. Can anyone help me to reach this concentration? Regards
16 October 2014 949 4 View
I used two restriction enzymes to cut my PCB1532 plasmid but I do not know when I run on gel how can I distinguish if my enzyme are not work properly. I cut this vector to use on fungal...
01 July 2014 8,399 14 View
I want to clone 10kb fragment to complete the complementation on fungi, could someone advise me how can I clone long fragment because I did before cloning 4kb fragment with classic methods. I do...
24 June 2014 7,013 3 View
Why did we use complementation inspite of how we examine how many copies of the gene mutated by southern blot?
04 May 2014 949 1 View
Also, I want to ask if I worked with agrobacterium I need to design a special plasmid for my fungus transformation or I can use the plasmid used with other species?
30 April 2014 9,486 2 View
DIG southern blot
08 April 2014 1,518 12 View
I am confused about selecting of enzyme for a Southern Blot and where exactly to cut it. I am working with knock out and used hygromycin selectable marker.
27 February 2014 659 1 View
