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As a research scientist, I constantly struggled with not being able to thoroughly describe my protocols in depth in the Materials & Methods section because of word limits. Now as an editor for...
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Is there a method to identify and measure truncated transcripts by RNA seq? How will the system distinguish between the full length and short transcripts?
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I am looking for someone who could provide me with astrocytes or glial cells from an animal model of autism. I would really appreciate if you could get in touch with me by sending me a message for...
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I want to use a primer extension assay to distinguish between a mutated and wild-type sequence using qPCR. What polymerase would be ideal for such an assay? I need something that stops dead in...
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I eluted my RNA after the RPE wash during the RNeasy kit and realized that my sample has carried over a lot of ethanol. How do I remove it? Should I re-precipitate or just let the ethanol...
31 August 2012 3,764 7 View
I am analyzing and sorting human fibroblasts with GFP transfected into them. They are not fixed---just trypsinized, harvested, washed and suspended in PBS. Recently we have started to see an odd...
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