Aditi Nadkarni

11 Questions 18 Answers 0 Followers

Questions related from Aditi Nadkarni

Do you have a detailed protocol that you could not describe well enough in the methods section of the article?

As a research scientist, I constantly struggled with not being able to thoroughly describe my protocols in depth in the Materials & Methods section because of word limits. Now as an editor for...

26 February 2016 7,233 4 View

What should be the size of ScriptSeq RNA Seq library after terminal tagging step?

I am making an RNA Seq library using ScriptSeq. This worked well the first time. But after experiencing failed libraries, I wanted to find out what stage I might be losing them at. So I conducted...

09 September 2015 4,354 0 View

Can a truncated transcript be identified or measured by RNA seq?

Is there a method to identify and measure truncated transcripts by RNA seq? How will the system distinguish between the full length and short transcripts?

08 August 2014 6,197 2 View

Which human cells grow best in serum-free or defined medium?

I am looking to conduct a proteomic study and need a cell line that can grow in serum free medium. Also is there a defined medium that is ideal for mass spec analysis such that the medium contents...

02 July 2014 8,791 6 View

Does anyone have experience with autism animal models?

I am looking for someone who could provide me with astrocytes or glial cells from an animal model of autism. I would really appreciate if you could get in touch with me by sending me a message for...

11 July 2013 3,475 1 View

Is there a high-throughput yeast crude (protein) extraction method?

I am looking for a high-throughput, preferably 96-well method to make crude protein extracts from yeast. Does anyone know of such a method? I am not a yeast biology person and have had limited...

28 May 2013 1,508 2 View

Which commercially available DNA polymerases lack flap endonuclease activity?

I need a DNA polymerase that does not have flap endonuclease activity for a differential priming assay that uses a tailed primer. I don't want the polymerase to excise the tail due to flap...

11 March 2013 3,641 2 View

Which RNA isolation kit yields good quality RNA for RNA seq analysis?

I am looking for an RNA kit to extract RNA from cells. This RNA will have to be high quality and good yield for RNA seq analysis. Suggestions? Thank you

31 January 2013 6,992 8 View

Primer extension assay to distinguish a mutated sequence from a wild-type sequence using qPCR

I want to use a primer extension assay to distinguish between a mutated and wild-type sequence using qPCR. What polymerase would be ideal for such an assay? I need something that stops dead in...

03 October 2012 1,384 6 View

Remove ethanol contamination from RNA sample

I eluted my RNA after the RPE wash during the RNeasy kit and realized that my sample has carried over a lot of ethanol. How do I remove it? Should I re-precipitate or just let the ethanol...

31 August 2012 3,800 7 View

Another cell population appears over time in a sample causing shift in flow cytometry read and problems in gating

I am analyzing and sorting human fibroblasts with GFP transfected into them. They are not fixed---just trypsinized, harvested, washed and suspended in PBS. Recently we have started to see an odd...

17 July 2012 6,727 17 View