Can someone provide me with protocols for enzyme inhibition assay and their kinetics?

More Olayinka Olarewaju's questions See All

How do I get protocol to isolate Dithymoquinone from Nigella saliva (black seeds)?

Good day scholars. Please I need detail protocol on how to isolate dithymoquinone from black seeds. I intended to buy dithymoquinone directly but what I kept getting is thymoquinone. Hence,...

02 December 2020 4,062 1 View

Why is Thymoquinone preferred over Dithymoquinone?

Thymoquinone and Dithymoquinone are found in black seeds (Nigella sativa). From their structures, dithymoquinone has 2 rings while thymoquinone has 1 ring. One would expect that dithymoquinone...

02 December 2020 303 2 View

Can I plot Bar Chart with significance levels represented by alphabets in R?

Good day house. Please can I plot Bar Chart with significance levels represented by alphabets in R? A file is attached to show the graphical representation of my question. I came across this bar...

15 October 2020 7,282 4 View

How do I determine Gestational Day 0 in Rats and Mice to start treatment?

Good day house. Please I intend to start exposure and treatment in Rats and Mice on the first day of pregnancy. So how do I determine the first day of pregnancy which is always designated as...

15 October 2020 7,402 4 View

'vina: command not found' error in virtual screening using autodock vina?

I am trying to carry out virtual screening using autodock vina. I have all the files necessary in my directory as suggested by literatures and tutorials. But when I run the command ./vina_vs.sh,...

22 September 2020 3,859 3 View

Anyone interested in Collaborating on Covid-19 Pandemic and Learners' Satisfaction?

The Covid-19 pandemic, described as unprecedented, has forced many educational institutions to switch from traditional or conventional (physical) mode of learning to a more flexible and digital...

15 June 2020 1,258 32 View

Is there R package and code to calculate MEAN +/- SEM?

Good day house. Please is there R package and code to calculate MEAN +/- SEM? I know of MEAN +/- SD, but I have not come across MEAN +/- SEM. Besides, is it possible to generate data set given...

05 April 2020 3,842 3 View

Can glass ionomer cement be used for cementing ceramic veneers as an alternative to resin cement?

I read an article which stated that resin modified glass ionomer cement (RMGIC) cannot be used for cementation of ceramic veneers due to development of hygroscopic expansion leading to fracture...

11 March 2020 3,684 4 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

11 April 2019 5,534 3 View

Which DNA Sequence Statistics should I do?

Good day house. I am working on Molecular Evolution and Phylogenetics of some organisms. Before conducting my Synonymous to Non Synonymous substitutions after Sanger Sequencing, I would need to...

08 April 2019 5,039 3 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

What is the best way to isolate Hydrolysable tannins on Sephadex column?

I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...

18 February 2021 1,455 3 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

FLICA assay not working?

Hi everyone, I am trying to perform the FLICA apoptosis assay with my MSCs. However, although the cells look healthy upon inspection and the viability is greater than 90% with cell counting, the...

17 January 2021 6,959 1 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View