Can someone provide me with protocols for enzyme inhibition assay and their kinetics?

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Could you please help me identify what might be wrong with my plaque assay?

I used Vero E6 cells and Mayaro virus. The virus was diluted in DMEM without FBS. The plaque media was 2X MEM, prepared with sodium bicarbonate and L-glutamine, adjusted to a pH of 4 with sodium...

03 June 2024 519 0 View

How can I turn the map I created (the .shp file) into an interactive map?

After collecting coordinates and mapping the road network, how do I then turn my created map into an interactive map

10 April 2024 3,659 1 View

What is the best agro waste used for mitigating corrosion? WHY?

Various agricultural residues have been employed for corrosion testing. Which specific agricultural residues are optimal for testing under a particular analysis?

28 December 2023 6,550 1 View

How do open a vcf file containing some sequences?

The vcf file i have contains some nucleotide sequences

22 August 2023 449 2 View

Is it possible to get a postdoctoral study after 5 years of obtaining a doctoral degree?

Most postdoctoral openings usually state that applicant's doctoral degree should not have exceeded 5 years before application. I need a possible list of University/Establishment that could admit...

03 July 2023 1,729 2 View

What can I use as a positive control for endothelial cell proliferation (MTT assay)?

Planning to run my assay this week but couldn't figure out positive control for MTT assay. I want to use cells without treatment as negative control while needing an agent or growth factor that...

12 March 2023 1,313 14 View

Is it possible for urea conc in a batch study to increase with time. The case that the urease enzyme should be hydrolyzing the urea?

I recently did a batch study with S.pasteurii and yeast extract plus some urea solution containing 20g/L urea. At 0hr sampling the urea conc was 13g/L, at 4 hrs sampling was 17g/L then it was...

18 July 2022 4,183 2 View

Can anyone help with a spectrophotometry method/protocol for determining dopamine level in rats' 🧠 brain?

Good day scholars. Please I need to determine the dopamine level in the brains 🧠 of experimental rats 🐀. I don't want to use ELISA; the alternative method I got requires spectroflurimeter which I...

10 June 2022 6,758 0 View

Can anyone help me with journals on quality financial reporting?

Journals that discuss the factors contributing to a quality financial reporting

14 September 2021 863 13 View

How do I determine B-Amyloid and NO in brain homogenate?

Good day scholars. Please can anyone help with detailed protocol(s) for the determination of Beta-Amyloid, dopamine, Nitric oxide (NO), Monoamine oxygenase A (MAO-A), and Acetylcholine esterase...

08 August 2021 2,357 1 View

What could be plastic adherent cells from PBMCs maintained in High Glucose DMEM 15% FBS ?

I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...

26 June 2024 3,664 1 View

Any tips for cell counting in 96-well plate?

Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...

22 June 2024 1,124 5 View

Why do I have an increasing concentration for increasing dilutions on spectroscopy even though they should all be the same final concentration?

Hello. We have a sample with an unknown concentration of polyphenols. We're diluting the sample in order to perform the Folin Ciocalteau method. 0.2 grams sample / 4 mL 70% methanol and 30%...

10 June 2024 2,134 3 View

Fibroblast trypsinization in Hanks medium with Ca and Mg?

We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....

28 May 2024 6,052 2 View

How to calculate the total phenolic contents (TPC) in plant extract sample?

How do I calculate the total polyphenol content in mg GAE/g. from the extract? Following is my data: Sample extract(stock): 0.01 gram in 1 ml Equation: y= 0.0078x - 0.0679, R2 =...

27 May 2024 1,911 2 View

How can we conduct tests using p-nitrophenyl substrates?

How to establish the calibration curve of PNP in relation to concentrations in a microplate and for measuring the activity of lipase, what do we use? Substrate stock: 20 mM p-NP palmitate in...

19 May 2024 1,286 0 View

Why the clear zone indicating amylase activity on agar plates dissappears after some hours?

I did qualitative assay on agar plates with starch as sole carbon source for checking amylase activity, once I added iodine the clear zone appeared arround the colonies but after some hours and...

13 May 2024 6,529 2 View

Assistant with TUNEL technique for A549 cells, switch from trypsinized cells to adherent cells?

Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...

07 May 2024 4,706 0 View

How do I isolate Lipase producing Fungi from spoilt Sheabutter and Ghee?

I prepared an enrichment media making use of the two samples spoilt Sheabutter and Ghee (differently) and it was cultured on Olive oil agar using pour plate method. The organisms were subcultured...

05 May 2024 5,795 1 View

How to analyze the percentages of covalently-bound and noncovalently-bound polyphenols during protein extraction?

I extracted protein from a roasted food sample using alkaline extraction followed by acid precipitation. Polyphenols were always coextracted since they were covalently bound with proteins during...

01 May 2024 7,192 2 View