I prepared an enrichment media making use of the two samples spoilt Sheabutter and Ghee (differently) and it was cultured on Olive oil agar using pour plate method. The organisms were subcultured to get pure isolates which was screened by plating them each on two different media; Tween80 agar and Tween80 with phenol red using a 5mm cork borer. Clear zones of hydrolysis was not observed and there was also no color change from red to yellow in the tween80 with phenol red media which would have indicated the presence of Lipase producing Fungi.
Hey Sofiyat, I would take a step wise approach: first isolation, second lipase activity confirmation. I’ll focus on isolation. If you have any evidence the fungi grow through the sample, then you could try break open the piece of butter or eventually cut a layer off with a sterile tool. Then you would take a small chunk of core butter and dilute it, probably with Tween80. Make serial dilutions (~3) and plate 100 uL of each in different media. Spread with a digralski loop or sterile beads. This should help isolating strains with different abundance in the original sample. I would expect these strains to grow very slow.
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