I have a very basic question. In genomic analysis we deal with coordinates. But I am little confused with the understanding about coordinates numbering system between positive and negative strand. I am aware of the 5'-3' orientations of positive and negative strands. But can someone explain me how coordinates are numbered for these strands. For example, If i have a position of nucleotide A at 432990 in Chromosome 11 positive strand, will the same position 432990 corresponds to the nucleotide T in the negative strand? Though I am not sure I guess there is no coordinates number for negative strand and the coordinate always refer to positive strand. However I expect someone to give me little more detail on this. Thanks.